BioBLU® Single-use Vessels from Eppendorf

(1) Breakthrough in large-scale production of iPSCs-derived exosomes to promote clinical applicationsFrontiers in Bioengineering and BiotechnologyAugust 24, 2023Xiaoxiao Chen, Ke Li, Jiangming Chen, Songwen Tan2D-Exos and has greater ability to repair cartilage defects (Yan and Wu, 2020). The ports reserved on the BioBLU 1c disposable bioreactor produced by Eppendorf were used to sample, inoculate and add media, add defoamers

(2) Mesenchymal and induced pluripotent stem cell–based therapeutics: a comparisonApplied Microbiology and BiotechnologyJuly 1, 2023Misha A. Teale, Samuel Schneider, Dieter Eibl, Christian van den Bos, Peter Neubauer, Regine Eiblbatch,Microcarriers,37*,6.1 (11)*,Rotondi et al. (2021) Mechanically driven:Stirred tank,15 mL–0.25 L,BioBLU® 0.3c SU,Eppendorf AG,hiPSCs,Perfusion,Spheroids,713,5.7 (7),Kropp et al. (2016) Mechanically driven:Stirred

(3) Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine MicroparticlesVaccines (Basel)May 12, 2023Pearl Akamine, José A. González-Feliciano, Ruth Almodóvar, Gloriner Morell, Javier Rivera, Coral M. Capó-Vélez, Manuel Delgado-Vélez, Luis Prieto-Costas, Bismark Madera, Daniel Eichinger, et al.production of gp145 was scaled up in a Finesse G3 Lab Bioprocess Controller (Thermo Scientific) using BioBLU 50 L single-use bioreactors (40 L working volume, Eppendorf). CHO-K1 cells were grown for 11 days in

(4) rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19Frontiers in ImmunologyMarch 30, 2023Ilias B. Esmagambetov, Ekaterina I. Ryabova, Artem A. Derkaev, Dmitry V. Shcheblyakov, Inna V. Dolzhikova, Irina A. Favorskaya, Daria M. Grousova, Mikhail A. Dovgiy, Vladimir V. Prokofiev, Andrey I. Gosudarev, et al.Epidemiology and Microbiology) and cultured in a BioFlo 320 bioreactor (Eppendorf, Germany) with a BioBLU 5c disposable vessel (Eppendorf, Germany) filled with Fibra-Cel matrix (Eppendorf, Germany). rAAV-P2C5-Fc

(5) Stem cell sources and characterization in the development of cell-based products for treating retinal disease: An NEI Town Hall reportStem Cell Research & TherapyMarch 29, 2023Ashley M. Fortress, Kiyoharu J. Miyagishima, Amberlynn A. Reed, Sally Temple, Dennis O. Clegg, Budd A. Tucker, Timothy A. Blenkinsop, George Harb, Thomas N. Greenwell, Tenneille E. Ludwig, et al.Suspension iPSC-RPE formulations are being produced in stirred-tank bioreactors including the Eppendorf BioBlu (3L) bioreactor. Suspension bioreactors are suitable for batch manufacturing allogeneic cell therapies

(6) rAAV expressing recombinant neutralizing antibody for the botulinum neurotoxin type A prophylaxisFrontiers in MicrobiologySeptember 27, 2022Artem A. Derkaev, Ekaterina I. Ryabova, Ilias B. Esmagambetov, Dmitry V. Shcheblyakov, Svetlana A. Godakova, Irina D. Vinogradova, Anatoly N. Noskov, Denis Y. Logunov, Boris S. Naroditsky, Alexander L. GintsburgEpidemiology and Microbiology) and cultured in a BioFlo 320 bioreactor (Eppendorf, Germany) with a BioBLU 5c disposable vessel (Eppendorf, Germany) filled with Fibra-Cel matrix (Eppendorf, Germany). pAAV–DJ

(7) Application of Ambr15 system for simulation of entire SARS‐CoV ‐2 vaccine production process involving macrocarriersBiotechnology ProgressJune 10, 2022Avital Jayson et al.preparation of a funnel for Fibra‐Cel insertion The VSV‐based vaccine production process is performed in the BioBLU® 5p Eppendorf single‐use bioreactors (Cino et al. 2011), pre‐packed with tens of thousands of Fibra‐Cels.

(8) Enabling Allogeneic T Cell-Based Therapies: Scalable Stirred-Tank Bioreactor Mediated ManufacturingFrontiers in Medical TechnologyMay 30, 2022Himavanth Gatla, Nicholas Uth, Yonatan Levinson, Ali Navaei, Alex Sargent, Senthil Ramaswamy, Inbar Friedrich Ben-Nunadded and was cultured until day 14. T Cell Activation and Expansion in a Stirred Tank Bioreactor The BioBlu single-use bioreactor vessel was set up according to the manufacturer's instructions (Eppendorf, 1386000300).

(9) SARS-CoV-2 spike antigen quantification by targeted mass spectrometry of a virus-based vaccineJournal of Virological MethodsMay 1, 2022Osnat Rosen et al.described (Barlev-Gross et al., 2021). 2.2 Vaccine samples The VSV-ΔG-spike vaccine was produced in BioBLU® 5p Eppendorf single-use bioreactors in Vero cell that were absorbed to Fibra-Cels. The medium from

(10) Manufacturing a chimpanzee adenovirus‐vectored SARS‐CoV‐2 vaccine to meet global needsBiotechnology and BioengineeringJanuary 1, 2022Carina C. D. Joe et al.Erlenmeyer flasks (Corning), with a working volume of 25–35 ml in a 125 ml flask unless otherwise stated. BioBlu 3c and 14c (Eppendorf) single‐use bioreactor vessels were used in accordance with the manufacturers'

(11) An Intranasal OMV-Based Vaccine Induces High Mucosal and Systemic Protecting Immunity Against a SARS-CoV-2 InfectionFrontiers in ImmunologyDecember 17, 2021Peter A. van der Ley, Afshin Zariri, Elly van Riet, Dinja Oosterhoff, Corine P. Kruiswijkat OD590 nm of ~1.8, the whole preculture was transferred to a 5l single-use bioreactor (Eppendorf BioBLU 3f) containing a total of 3.2 l medium volume. The culture conditions were controlled (Eppendorf BioFlo 320

(12) In-Line Monitoring of Downstream Purification Processes for VSV Based SARS-CoV-2 Vaccine Using a Novel TechniqueBioTech (Basel)November 3, 2021Arik Makovitzki et al.products. 2. Materials and Methods 2.1. Virus Upstream Production The rVSV-∆G-spike vaccine was produced in BioBLU® 5p Eppendorf single-use bioreactors (Hamburg, Germany) [6] or in multi trays (MT) (NUNC EasyFill Cell

(13) Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine ApplicationsBioengineering (Basel)May 17, 2021Diogo E.S. Nogueira, Joaquim M.S. Cabral, Carlos A.V. Rodriguessystems Three-blade mL–2.0 L Table®2.SU Examples of single-use stirred-tank with successful use for 600 stem cell culture. UniVessel Bioreactor BioBLU® Mobius® CellReady Company Impeller Working Volume Kropp et al. have described the expansion of hiPSCs

(14) Lentiviral Vector BioprocessingVirusesFebruary 9, 2021Christopher Perry, Andrea C. M. E. Rayat; Corning HyperStack 6000–18,000 Cytiva * WAVE 10–200 L Cytiva * Xcellerex XDR 4.5–2000 L Eppendorf BioBLU 5p Up to 180,000 Pall Allegro STR 20–2000 L (12–36 layers) cm2 m2 ; Pall iCELLIS 0.53–4 1

(15) Does the BioBLU 0.3f single-use scale to the BioFlo® 320 reuseable bioreactor on a matched volumetric oxygen mass transfer coefficient?World Journal of Microbiology and BiotechnologyJanuary 4, 2021Williams Olughu, Duncan Galbraith, Cillian Paget, Steve Ruscoe, Josh Smith, Alison Masoninterference from metabolic activity, which also reduced experimental complexity. Materials and methods The BioBLU® 0.3f single-use and the BioFlo® 320 reusable bioreactors (Eppendorf AG, Germany) were used in this

(16) Challenges & trends in stem cell-derived extracellular vesicle bioprocessingCell and Gene Therapy InsightsNovember 12, 2020Jorge L Escobar IviricoSpeaking of stirred-tank bioreactors for upstream production process, Eppendorf ’s portfolio includes BioBLU® Single-Use Vessels of up to 40 L of working volume for cell culture applications. Another critical aspect to consider is

(17) Separation of influenza virus-like particles from baculovirus by polymer-grafted anion exchangerJournal of separation scienceJune 1, 2020Reiter K, Pereira Aguilar P, Grammelhofer D, Joseph J, Steppert P, Jungbauer A.clumping. 2.2.2 Benchtop bioreactor cultivations Production was performed in a 10 L single use bioreactor (BioBLU 10c, Eppendorf) equipped with one pitched‐blade impeller (3 blades; 45°). The temperature was set to

(18) Inhibition of SYK kinase does not confer a pro-proliferative or pro-invasive phenotype in breast epithelium or breast cancer cellsOncotargetApril 7, 2020Lamb DJ, Rust A, Rudisch A, Glüxam T, Harrer N, Machat H, Christ I, Colbatzky F, Wernitznig A, Osswald A, et al.tumour spheroids were cultivated in a DASbox® Mini Bioreactor System (Eppendorf, #76DX04CCSU) utilizing BioBLU® 0.3 Single-Use Vessels (Eppendorf) essentially as described [19]. The culture medium was RPMI (without

(19) An additive manufacturing approach to bioreactor design for mesenchymal stem cell cultureBiochemical Engineering JournalApril 1, 2020Akinlolu Oyekunle Oluseun Odeleye, Timothee Baudequin, Chih-Yao Chui, Zhanfeng Cui, Hua Yeto 6-fold increase in cell number [7], [8]. Other suspension stirred systems include the Celligen® BioBLU (Eppendorf, Hauppauge, NY, USA), DASGIP Parallel Bioreactor system, PADReactor (Pall Life Sciences),

(20) Benchmarking of Scale-X Bioreactor System in Lentiviral and Adenoviral Vector ProductionHuman Gene TherapyMarch 1, 2020Hanna M. Leinonen, Saana Lepola, Eevi M. Lipponen, Tommi Heikura, Tiina Koponen, Nigel Parker, Seppo Ylä-Herttuala, Hanna P. Leschdeveloped, such as Celligen (NewBrunswick Scientific),15 CellCube (Costar),15 packed-bed bioreactors (BioBLU; Eppendorf),16 and microcarrier-based bioreactors.17 Among the latest inventions is scale-X bioreactor

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BioBLU® Single-use Vessels from Eppendorf

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BioBLU® Single-use Vessels from Eppendorf

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